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以受体杨树品种‘107号’和转基因杨树‘18-1’的一年生枝条为材料,采用Hoagland营养液水培方法,检测二者植株生长以及根、茎、叶中Na+和K+含量变化的结果表明,在含100mmol·L-1NaCl的Hoagland营养液中,二者的生长速度都受到明显抑制,但后者的受抑程度较小;叶中Na+含量呈持续增加趋势,9d后其叶中的Na+含量显著高于前者。在二者叶片枯萎程度相同的情况下,‘18-1’叶中的Na+含量是‘107号’的1.6倍左右。在4mol·L-1NaCl溶液中,它们的表皮细胞死亡率分别为42%和97%。说明后者比前者有更高的耐盐能力。
The annual shoots of the poplar cultivar ’107’ and the transgenic poplar ’18-1’ were used as the materials, and the changes of Na + and K + contents in roots, stems and leaves of the two plants were detected by Hoagland nutrient solution hydroponics. The results showed that in the Hoagland nutrient solution containing 100 mmol·L-1 NaCl, the growth rate of both was significantly inhibited, but the inhibition of the latter was less; the content of Na + in the leaves tended to increase continuously. After 9 days, In the Na + content was significantly higher than the former. Under the same degree of leaf wilting, Na + content in ’18-1’ leaves was about 1.6 times that of ’107’. In the 4 mol·L-1 NaCl solution, their epidermal cell death rates were 42% and 97%, respectively. The latter shows a higher salt tolerance than the former.