论文部分内容阅读
目的 调查在 EL ISA方法检测血清 HBs Ag阴性飞行学员中 ,血清 HBV- DNA的检出情况。 方法 用 EL ISA法检测 2 2 2 4名应招飞行学员血清 HBs Ag。其中阴性者进一步检测抗 - HBs、抗 - HBc、HBe Ag、抗 - HBe,并用聚合酶链反应 (PCR)检测 HBs Ag阴性者 HBV- DNA。 结果 在 2 2 2 4名应招飞行学员中 HBs Ag阳性 15 8例 ,阳性率 7.1% ;HBs Ag阴性者中 ,除单项抗 - HBs阳性血清模式外 ,其他各模式均有不同例数的人检测到 HBV- DNA。 结论 本研究表明 ,仅检测血清中 HBs Ag的表达状况 ,不足以将所有感染有 HBV者筛选出去 ,应由灵敏度更高的分子生物学方法取代
Objective To investigate the detection of serum HBV DNA in EL-ISA serum HBs Ag-negative flight test students. Methods The EL ISA method was used to detect the serum HBs Ag of 22 224 students who should be invited to fly. Among them, anti - HBs, anti - HBc, HBe Ag, anti - HBe were further detected by negative test and HBV - DNA was detected by polymerase chain reaction (PCR). Results There were 15 8 HBs Ag positive cases among the 2 224 recruited pilots, with a positive rate of 7.1%. Among the HBs Ag negative patients, all the other models had different numbers of human tests except for the single anti-HBs positive serotypes To HBV-DNA. Conclusions This study shows that detection of HBsAg expression in serum alone is not sufficient to screen all patients infected with HBV and should be replaced by a more sensitive molecular biology approach