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以现代月季(Rosa hybrida)抗感不同的杂交后代作为研究对象,测定黑斑病菌(Diplocarpon rosae)侵染后对叶片表皮细胞结构的影响。经扫描电镜观察,在病菌未侵染时叶片表皮细胞均正常,表皮有气孔分布,气孔椭圆形且饱满。但接种病菌6d后,抗病后代中的感病叶片表皮均有大量菌丝分布,有少数菌丝进入气孔内部;感病后代中的感病叶片表皮肿胀、气孔下陷、孢子附着在叶片表面。通过透射电镜观察,未接种月季黑斑病菌时叶片表皮细胞正常,接种病原菌之后抗病后代叶肉细胞内含较大、较多的嗜锇颗粒,叶绿体由椭圆形变为近圆球形,类囊体形态均一性降低,排列松散,定向发生紊乱;感病后代细胞内叶绿体整体瓦解,只保留了液泡和细胞核,且出现较多的空泡。随着病菌的进一步侵染,细胞质完全降解,导致细胞死亡。
The hybrid progenies resistant to Rosa hybrida were used as research objects to determine the effect of Diplocarpon rosae infection on the epidermal cell structure of leaves. By scanning electron microscopy, the leaf epidermal cells were normal in the non-infected bacteria, the epidermis stomatal distribution, stomatal oval and full. However, after inoculation of pathogen for 6 days, a large amount of mycelium was distributed in the infected epidermis of diseased offspring, and a few mycelium entered the stoma interior. The susceptible leaf epidermis swollen, stomatal subsidence and spore attached to the leaf surface. The results showed that the leaf epidermal cells were non-inoculated with Alternaria septempunctata and the mesophyll cells contained larger and more osmiophilic granules in the diseased progenies after inoculation with pathogenic bacteria. The chloroplasts changed from oval to nearly spherical, Homogeneity decreased, loosely arranged, and disordered orientation. The chloroplasts in the offspring of the susceptible cells disintegrated as a whole, leaving only vacuoles and nuclei and more vacuoles. With further bacterial infection, the cytoplasm completely degrades, resulting in cell death.