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分光光度法测定药物含量,常用标准曲线法、比较法和吸收系数(_(1cm)~(1％))法三种。后者,在海内外药物分析中被广泛应用。该法计算含量,是将样品精取一定量,用适当溶剂溶解或稀释至适当浓度,在最大吸收波长(λmax)处测定吸收度(A),将其换算成样品的吸收系数([E_(1cm)~(1％)]样),再与已知吸收系数([E_(1cm)~(1％)]标)比较.但如何通过实验中测得的吸收度、稀释倍数及已知吸收系数来计算供试品百分含量、注射液及片剂含量为其标示量的百分率,则是此法的难点。我们在多年的教学和药房分析工作中,总结出简单可行的计算方
Spectrophotometry determination of drug content, commonly used standard curve method, comparative method and the absorption coefficient (_ (1cm) ~ (1%)) Act of three. The latter, is widely used in drug analysis at home and abroad. The method calculates the content by taking a certain amount of the sample, dissolving or diluting it with a suitable solvent to an appropriate concentration, measuring the absorbance (A) at the maximum absorption wavelength (λmax) and converting it into the absorption coefficient ([E_ 1cm) ~ (1%)]), and compared with the known absorption coefficient ([E_ (1cm) ~ (1%)] standard), but how the absorption measured in the experiment, the dilution factor and the known absorption Coefficient to calculate the percentage of the test sample, injection and tablet content as the percentage of its labeled amount is the difficulty of this method. In years of teaching and pharmacy analysis, we have come up with simple and feasible calculations