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目的:研究锌转运体在白血病各阶段的变化规律及与白血病疾病发展过程的相关性,探讨锌浓度及其转运体对造血调控作用的影响。方法:将实验对象分为急性髓系白血病组、慢性粒细胞白血病组、正常组、细胞株组;采用半定量RT-PCR的方法测定人类锌铁调控蛋白(human ZRT/IRT-like protein,hZIP1)及阳离子转运促进因子1(cation diffusion facilitator,CDF,又称ZnT1)mRNA的表达;应用火焰原子吸收法测定骨髓上清中的锌含量。结果:hZIP1基因在骨髓单个核细胞上阳性表达,但在急、慢性白血病的各个阶段的表达均无显著性差异(P>0.05);ZnT1基因在骨髓单个核细胞上阳性表达,初治、复发组白血病患者ZnT1表达量明显高于正常对照和缓解组(P<0.05);在K562、HL60细胞株中的实验结果与临床标本的实验结果一致;急性白血病初治组和复发组骨髓上清锌含量明显高于缓解组和正常组(P<0.05)。结论:hZIP1基因与细胞内外的锌水平调节有关,但与白血病时细胞内外锌含量变化可能无明显关系;ZnT1与白血病骨髓单个核细胞中锌水平调节密切相关,是调节细胞内外锌含量的重要基因;ZnT1表达水平降低、骨髓上清锌含量降低提示病情好转。
OBJECTIVE: To study the changes of zinc transporter in leukemia stages and its correlation with the development of leukemia, and to explore the effect of zinc concentration and its transporter on hematopoietic regulation. Methods: The experimental group was divided into acute myeloid leukemia group, chronic myelogenous leukemia group, normal group and cell line group. The mRNA expression of human ZRT / hTNR1 ) And cation diffusion facilitator (CDF, also known as ZnT1) mRNA in bone marrow. The content of zinc in bone marrow supernatant was determined by flame atomic absorption spectrometry. Results: The hZIP1 gene was positively expressed on bone marrow mononuclear cells, but there was no significant difference in all stages of acute and chronic leukemia (P> 0.05). The expression of ZnT1 gene on bone marrow mononuclear cells was positive, The expression level of ZnT1 in patients with leukemia was significantly higher than that in the normal control and remission groups (P <0.05). The experimental results in K562 and HL60 cell lines were consistent with those in clinical specimens. Content was significantly higher than the remission group and normal group (P <0.05). CONCLUSION: The hZIP1 gene is related to the regulation of intracellular and extracellular zinc levels, but may not be related to the changes of intracellular zinc levels in leukemia. ZnT1 is closely related to the regulation of zinc levels in leukemia bone marrow mononuclear cells and is an important gene regulating the zinc content in cells ; ZnT1 expression decreased, decreased bone marrow zinc content prompted improvement.