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采用微生物培养、BIOLOG碳素利用法和土壤酶活性测定等方法,分析了日光温室不同地下灌溉制度下黄瓜根际土壤中微生物活性及功能多样性.结果表明:根际土壤微生物生物量C、N含量、基础呼吸、代谢熵、AWCD值、Shannon指数和McIntosh指数随灌水量的增加呈先升高后下降的趋势;在0.8Ep(Ep为20 cm标准蒸发皿蒸发量)灌溉水平下,I2处理(灌水周期8 d)根际土壤微生物生物量C、N含量、基础呼吸、代谢熵、AWCD值、Shannon指数和McIntosh指数显著高于I1处理(灌水周期4 d).0.8Ep处理下,细菌、放线菌、自生固氮菌数量及脲酶、磷酸酶、蔗糖酶、过氧化氢酶和多酚氧化酶活性显著高于其他2个灌水量处理(0.6Ep和1.0Ep);I2处理的细菌和自生固氮菌数量、脲酶、磷酸酶和蔗糖酶活性显著高于I1处理,放线菌数量、过氧化氢酶和多酚氧化酶活性与I1处理差异不显著,而真菌数量显著低于I1处理.I20.8Ep处理使黄瓜根际土壤中微生物代谢活性和微生物群落功能多样性升高,微生物区系得以改善,土壤酶活性提高,促进黄瓜生长.
The microbial activities and functional diversity of cucumber rhizosphere soils under different underground irrigation systems in sunlight greenhouse were analyzed by means of microbial culture, BIOLOG carbon utilization and soil enzyme activity determination.The results showed that the rhizosphere soil microbial biomass C, N The content, basal respiration, metabolic entropy, AWCD value, Shannon index and McIntosh index increased first and then decreased with the increase of irrigation amount. Under the irrigation level of 0.8Ep (Ep was 20 cm standard pan evaporation), I2 treatment (8 days after irrigation), microbial biomass C, N, basic respiration, metabolic entropy, AWCD, Shannon index and McIntosh index of rhizosphere soil were significantly higher than that of I1 (irrigation cycle 4 d) The number of actinomycetes, autotrophic Azotobacter and the activities of urease, phosphatase, sucrase, catalase and polyphenol oxidase were significantly higher than those of the other two treatments (0.6Ep and 1.0Ep) The number of diazotrophs, urease, phosphatase and invertase activity were significantly higher than that of I1, the number of actinomycetes, the activity of catalase and polyphenol oxidase were not significantly different from that of I1, but the number of fungi was significantly lower than that of I1 0.8Ep treatment increased soil microbial metabolic activity and microbial community functional diversity in cucumber rhizosphere soil, improved microbial flora, enhanced soil enzyme activity, and promoted cucumber growth.