全细胞差减筛选法是近年来在细胞筛选的基础上发展起来的一项筛选技术,其利用成对的细胞,即两种不同状态的细胞对噬菌体展示文库进行差减筛选,主要用于筛选新的抗原表位、受体、配体、新型疫苗、肿瘤细胞的靶向活性肽、靶向基因载体及受体或酶的激动剂或抑制剂等。本文就其筛选的原理、优缺点、优化及其在噬菌体展示文库筛选中的应用作一综述。 The whole cell subtraction screening method is a screening technique developed in recent years based on cell selection. It uses two pairs of cells, that is, cells of two different states to perform differential screening on the phage display library and is mainly used for screening New epitopes, receptors, ligands, novel vaccines, targeted active peptides for tumor cells, agonists or inhibitors of targeted gene vectors and receptors or enzymes, and the like. This review summarizes the principles, advantages, disadvantages, and optimizations of its screening and its application in the screening of phage display libraries.