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目的:建立妇安消疹洗液中落新妇苷的含量测定方法。方法:采用高效液相色谱法,色谱柱:Diamonsil-C_(18)柱(250mm×4.6 mmn,5μm);流动相:甲醇-0.1%冰醋酸溶液(39∶61);检测波长:291 nm,流速:1.0 ml·min~(-1),进样量:10μl,柱温:30℃。结果:落新妇苷在0.05~1.60 mg·ml~(-1)的范围内线性关系良好(r=0.999 8),平均回收率为97.7%,RSD为1.2%(n=6)。结论:所建方法简便快捷,专属性强,适用于妇安消疹洗液中落新妇苷的含量测定。
Objective: To establish a method for the determination of astilbin in the women’s anti-erosive lotion. METHODS: High performance liquid chromatography (HPLC) was used with a Diamonsil-C 18 column (250 mm × 4.6 mmn, 5 μm). The mobile phase consisted of methanol-0.1% glacial acetic acid (39:61) Flow rate: 1.0 ml · min ~ (-1), injection volume: 10μl, column temperature: 30 ℃. Results: Astilbin had a good linearity (r = 0.999 8) in the range of 0.05-1.60 mg · ml -1 with an average recovery of 97.7% and RSD of 1.2% (n = 6). Conclusion: The method is simple, rapid and specific. It is suitable for the determination of astilbin in the decoction of Fu-An.