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[目的 ]对已知的宁化县斑点热群立克次体疫源地进行全面系统 SFGR感染情况调研。 [方法 ]采用微量室温补体结合法 (m CF)和间接免疫荧光 (IFA)法 ;鸡胚卵黄囊感染法并用 m CF、PCR/ RFL P分子生物学技术进行鉴定。[结果 ]人群血清阳性率为 12 .7% (12 1/ 95 2 )。宿主动物 SFGR感染 ,抗体阳性率 0～ 5 5 .6 %不等。鉴定分类各种蜱5 6 0 0余只 ,共 6属 13种。从野兔、麂体表寄生的越原血蜱中分离出 2株 SFGR病原体 ,鉴定为西伯利亚立克次体。 [结论 ]从人群、宿主动物、媒介蜱、流行病学和病原学上进一步证实福建省宁化县存在斑点热群立克次体自然疫源地
[Objective] To investigate the status of systemic SFGR infection in the known epidemic area of Rickettsia plague in Ninghua County. [Method] Micro-room temperature complement fixation method (m CF) and indirect immunofluorescence (IFA) method were used. Chicken embryo yolk sac infection method was identified by m CF, PCR / RFL P molecular biological techniques. [Results] Seroprevalence of the population was 12.7% (12 1/95 2). The host animal SFGR infection, antibody positive rate of 0 ~ 55.6% range. Identification of various ticks more than 5600, a total of 6 genera and 13 species. Two strains of SFGR were isolated from the haemaphysal parasites parasitized by rabbits and muntjac, and identified as Siberian rickettsia. [Conclusion] The natural epidemic foci of Rickettsia plague in Ninghua County, Fujian Province, were further confirmed from population, host animals, ticks, epidemiology and etiology