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目的:探讨低电压电化学疗法对头颈部肿瘤的治疗意义。方法:于裸鼠下颌皮下接种人头颈部鳞状上皮细胞癌YCU-N861细胞系细胞,制成移植瘤模型。局部注射博来霉素(BLM)20μg/只,继以50V、100V的电压刺激(EP),观察肿瘤生长情况,并测量其体积。9d后取肿瘤组织,常规苏木精-伊红染色及免疫组织化学染色后观察。结果:BLM和EP联合应用能明显抑制肿瘤细胞的增殖,联合治疗组(BLM+EP)与BLM组、EP组和空白对照组比较,均差异有统计学意义(均P<0.01);BLM+EP组肿瘤生长发育迟缓,明显受到抑制,组织学表现为坏死出血征象,有丝分裂指数和免疫组织化学Ki67染色标记指数分别为9.90±3.48、2.28±2.61,与BLM组(34.33±6.60、6.41±3.59)、EP组(58.75±8.87、8.85±3.25)及对照组(73.25±11.95、11.10±3.67)比较,差异有统计学意义(均P<0.01)。结论:低电压电化学疗法在头颈部肿瘤的治疗方面具有广阔的应用前景。
Objective: To investigate the therapeutic effect of low-voltage electrochemical therapy on head and neck cancer. Methods: The xenografts of human head and neck squamous cell carcinoma cell line YCU-N861 were inoculated subcutaneously into the mandible of nude mice to make a xenograft tumor model. Local injection of bleomycin (BLM) 20μg / only, followed by 50V, 100V voltage stimulation (EP), observe the tumor growth, and measure the volume. After 9 days, the tumor tissues were taken out and stained with hematoxylin-eosin and immunohistochemical staining. Results: The combination of BLM and EP could significantly inhibit the proliferation of tumor cells. There was significant difference between BLM + EP group and BLM group, EP group and blank control group (all P <0.01) The tumor growth retardation in EP group was significantly inhibited, and the histological findings showed signs of necrotic hemorrhage. The mitotic index and Ki67 staining index were 9.90 ± 3.48 and 2.28 ± 2.61, respectively, which were significantly higher than those in BLM group (34.33 ± 6.60,6.41 ± 3.59 ), EP group (58.75 ± 8.87, 8.85 ± 3.25) and control group (73.25 ± 11.95, 11.10 ± 3.67), the difference was statistically significant (all P <0.01). Conclusion: Low-voltage electrochemical therapy has broad application prospects in the treatment of head and neck cancer.