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目的:观察金丝桃苷(hyperin,Hyp)对四氯化碳(CCl4)致小鼠急性肝损伤的保护作用。方法:将180只雌雄小鼠分为3批进行实验。每批小鼠随机分为正常对照组,CCl4模型对照组,联苯双酯阳性对照组,Hyp高、中、低(50,25和12.5 mg.kg-1)3个剂量组。各给药组均连续给药7 d,末次给药1 h后,给药组与模型组均ip 0.1%CCl4油剂(0.1 mL/10 g体重)。中毒小鼠16 h后眼球取血,测定血清中谷丙转氨酶(ALT)和谷草转氨酶(AST)水平,并迅速处死小鼠取肝,检测肝组织中丙二醛(MDA)含量和超氧化物歧化酶(SOD)活力,并行肝切片镜检观察各组肝脏的病理变化。结果:CCl4模型组小鼠肝功能明显异常,MDA含量明显升高,SOD活力明显下降。Hyp组能明显改善CCl4中毒小鼠的肝功能,使其ALT和AST显著降低,高、中、低剂量组降酶率分别为89.6%,86.5%和78.3%;肝组织中SOD活力明显升高,MDA含量降低;CCl4模型组小鼠肝脏损伤严重,Hyp高剂量组的肝病理改变明显轻于CCl4模型组。结论:Hyp对CCl4急性肝损伤小鼠有较好的保肝降酶作用。
Objective: To observe the protective effect of hyperin (Hyp) on acute liver injury induced by carbon tetrachloride (CCl4) in mice. Methods: 180 male and female mice were divided into three batches for experiments. Each batch of mice were randomly divided into normal control group, CCl4 model control group, biphenylester positive control group, Hyp high, medium and low (50,25 and 12.5 mg.kg-1) three dose groups. Each administration group was administered continuously for 7 days. After the last administration for 1 hour, 0.1% CCl4 oil (0.1 mL / 10 g body weight) was administered to both the administration group and the model group. Twenty-four hours after the poisoning, blood was collected from the eyeballs, and serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were measured. The mice were sacrificed and the liver was taken. Malondialdehyde (MDA) content and superoxide dismutase Enzyme activity (SOD), liver biopsy and liver biopsy were observed pathological changes. Results: The liver function of mice in CCl4 model group was significantly abnormal, MDA content was significantly increased, SOD activity was significantly decreased. The Hyp group could significantly improve the hepatic function of CCl4-poisoned mice and significantly reduce the ALT and AST levels, and the reductase rates were 89.6%, 86.5% and 78.3% in the high, medium and low dose groups, respectively. The SOD activity in the liver tissue was significantly increased , MDA content decreased; CCl4 model mice liver injury severe Hyp high dose group of liver pathology was significantly lighter than the CCl4 model group. Conclusion: Hyp has a good hepatoprotective effect on CCl4-induced acute liver injury in mice.