目的建立棉花花总黄酮片中总黄酮、金丝桃苷、异槲皮素的测定方法。方法利用紫外分光光度计,以芦丁为对照品测定总黄酮,HPLC同时测定黄酮类化合物金丝桃苷和异槲皮素。结果 3批棉花花总黄酮片中总黄酮分别为71.6、73.5、75.2 mg/片,金丝桃苷分别为4.28、4.35、4.23 mg/片,异槲皮素分别为18.55、18.73、18.31 mg/片。结论该方法专属性强,灵敏度高、重现性好,可用于棉花花总黄酮片中黄酮类化合物的测定。 Objective To establish a method for the determination of total flavonoids, hyperoside and isoquercetin in cotton flaxseed tablets. Methods The total flavonoids were determined by UV spectrophotometer with rutin as reference substance and the simultaneous determination of hyperin and isoquercetin by HPLC. Results The results showed that the total flavones in the three flavonoids tablets were 71.6, 73.5 and 75.2 mg / tablet, respectively, the values ​​of hyperoside were 4.28, 4.35 and 4.23 mg / tablet, isoquercitrin were 18.55, 18.73 and 18.31 mg / sheet. Conclusion The method is specific, sensitive and reproducible. It can be used for the determination of flavonoids in the total flavonoids of cotton flower.