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目的分析青海省2007-2011年各类艾滋病病毒(HIV)检测筛查实验室送检的HIV抗体呈阳性反应样品的确证符合率,为提高省内各类机构的HIV检测工作质量提供科学依据。方法采用酶联免疫吸附试验(ELISA)双抗原夹心法,对送检样品进行复检,对呈阳性反应的样品用蛋白免疫印迹试验(WB)进行确证,使用Excel 2007和SPSS 16.0统计软件整理和分析试验结果。结果 1 065份送检样品中,有672份复检试验呈阳性反应,阳性率为63.1%。2007-2011年间HIV确证阳性率分别为58.2%、418%、68.9%、71.9、81.1%。四类筛查实验室中,疾控机构筛查实验室的HIV确证阳性率为87.0%,大型医疗机构筛查实验室为76.3%,其他检测机构为66.3%,采供血机构筛查实验室为26.6%。结论全省各年HIV确证阳性率逐年提高,各类机构HIV筛查实验室的检测水平仍需加强,同时要严格遵循《全国艾滋病检测技术规范(2009年版)》的要求开展HIV检测工作。
Objective To analyze the exact coincidence rate of HIV antibody positive samples submitted by various HIV testing and screening laboratories in Qinghai from 2007 to 2011, and provide a scientific basis for improving the quality of HIV testing in various agencies in the province. Methods The double-antigen sandwich method was used for the retesting of samples. The positive samples were confirmed by Western blotting (WB). The data were analyzed by Excel 2007 and SPSS 16.0 software. Analyze the test results. Results A total of 1 065 samples were tested, and 672 retesting tests were positive, with a positive rate of 63.1%. The positive rates of HIV confirmatory tests in 2007-2011 were 58.2%, 418%, 68.9%, 71.9 and 81.1% respectively. Among the four types of screening laboratories, the HIV-positive positive rate of CDC screening laboratories was 87.0%, that of large-scale medical institutions screening laboratories was 76.3% and that of other testing organizations was 66.3%. The blood sampling and screening laboratories 26.6%. Conclusions The positive rate of HIV positive certificates in the whole province has been increasing year by year. The detection level of HIV screening laboratories in all kinds of institutions still needs to be strengthened. At the same time, it is necessary to strictly follow the requirements of “National AIDS Testing Technical Specification (2009 Edition)” for HIV testing.