论文部分内容阅读
目的 观察能量限制后不同饮食模式重饲对骨参数的影响及潜在影响因素.方法 8w龄雄性SD大鼠分为普通饮食(NC4、NC12)组、能量限制(R4)组、限食后普通饮食重饲(RN)组、限食后高脂饮食重饲(RH)组、高脂饮食(HF)组.实验4w、12w检测股骨、脊柱及全身骨参数、体质量、血清甘油三酯(TG)及脂联素水平.结果 (1)限食使R4组骨参数明显降低(P＜0.05);重饲后,RN组各部位骨参数恢复程度较RH组显著(P＜0.05);HF组各骨参数较NC12组均显著升高(P＜0.05).(2) R4组脂联素水平显著升高,TG水平明显降低(P＜0.01);重饲后RH组脂联素及TG水平均高于RN组(P＜0.05);HF组脂联素水平明显低于其他组,TG水平高于NC12组,但低于RN及RH组(P＜0.01).(3)全身骨密度与体质量、TG成正相关,r值分别为0.96,0.643(P＜0.01);与脂联素呈负相关(r=-0.818,P＜0.01).进一步行回归分析显示TG、脂联素是影响骨密度的主要因素(R2=0.932,P＜0.001.).结论 重饲可以部分修复热卡限制导致的骨损害,开放不同饮食模式骨参数恢复程度不同.TG和脂联素是影响骨密度的重要因素.“,”Objective To investigate the effect of refeedings with different dietary patterns after calorie restriction on bone parameters and potential influence factors.Methods Male SD rats,aged 8 weeks,were divided into normal chow (containing NC4 and NC12) group,calorie restriction (R4) group,refeeding with normal chow after calorie restriction (RN)group,refeeding with high-fat diet after calorie restriction (RH) group,and high-fat diet (HF) group.Bone parameters of femur,spine and the whole body skeleton were detected at the end of 4 and 12 weeks,along with other indicators (body weight,serum adiponectin and triglyceride levels).Results (1) All the parameters decreased in R4 group (vs.NC4,P＜0.05).After refeeding,the recovery degree of bone parameters in RN group was higher than that in RH group (P＜0.05).Bone parameters in HF group were significantly elevated (vs.NC12,P＜0.05).(2) The level of serum adiponectin was markedly increased and TG level was decreased in R4 group (vs.NC4,P＜0.01).After refeeding,serum adiponectin and TG in RH group were significantly increased compared with those in RN group (P＜0.05).Among all groups,the level of serum adiponectin in HF group was the lowest,while the TG in HF group was significantly higher than that in NC12 group (P＜0.01),but obviously lower than that in RN and RH groups (P＜0.01).3.There was a positive correlation between the whole body bone mineral density,body weight and serum TG (r values were 0.96 and 0.643 respectively,P＜0.01).In contrast,an inverse correlation was found between the whole body bone mineral density and serum adiponectin (r=-0.818,P ＜ 0.01).In the linear regression analysis,both serum TG and adiponectin were main influential factors for bone density (R2=0.932,P＜0.001).Conclusion The study shows that refeeding can partially reverse the deleterious effects of caloric restriction on bone health,and different dietary patterns lead to different recovery degree of bone parameters.Serum TG and adiponectin play an important role in regulating bone mineral density.