论文部分内容阅读
Objective:To observe the feasibility of gene silencing of MAT1 gene by small interference RNA in human pancreatic cancer cells.Methods:BxPC-3 cells were transfected using chemically synthesized double stranded RNA formulated with liposome.Gene expression of MAT1 was assayed by RT-PCR and Western blot respectively.The cell proliferation assay was carded out by counting alive cells after Trypan blue exclusion.The cells invasion ability was determined by Boyden chamber model.Results:The MAT1 mRNA and protein expression of BxPC-3 cells were significantly down-regulated by small interference RNA compared with the control groups.The expression of MAT1 mRNA was reduced by 55.2% and 64.3% in 24 h and 48 h respectively (P＜0.01).The cell proliferation and invasion ability of BxPC-3 cell were significantly inhibited (P＜0.01).Conclusion:The results suggest that gene silencing of MAT1 by siRNA can inhibit the cell proliferation and invasion of BxPC-3 cells,which may be a target in the gene therapy of human pancreatic cancer.