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目的:探讨巴戟天寡糖(MOO)对鸡胚绒毛尿囊膜(CAM)血管生成的影响。方法:运用血清药理学的方法制备含药血清。60只鸡胚随机分为MOO低、中、高剂量组及生理盐水(NS)阴性对照组、空白血清对照组、碱性成纤维细胞生长因子(bFGF)阳性对照组,每组10只。孵育7d后建立CAM模型,将NS、空白血清、bFGF(2500U.mL-1)、MOO3种剂量含药血清分别加在CAM表面的载体上,继续孵育3d后制备CAM标本,观察血管生成表现,并进行新生血管计数。结果:MOO各剂量组与bFGF组血管生成表现明显优于NS组与空白血清组。MOO各剂量组新生血管数目较空白血清组均明显增加(P<0.05),但药效均弱于bFGF组(P<0.05)。与MOO低剂量组相比,中、高剂量组新生血管数显著增加(P<0.05),但两者之间无显著性差异。NS组与空白血清组新生血管数目之间无显著性差异。结论:MOO可促进鸡胚绒毛尿囊膜的血管增生,具有一定的促血管新生作用。
Objective: To investigate the effect of Morinda oligosaccharides (MOO) on the angiogenesis of chick chorioallantoic membrane (CAM). Methods: Using serum pharmacology method to prepare drug-containing serum. Sixty embryos were randomly divided into MOO low, middle and high dose group and NS negative control group, blank serum control group and basic fibroblast growth factor (bFGF) positive control group, with 10 mice in each group. The CAM model was established after 7 days of incubation. NS, blank serum, bFGF (2500U.mL-1) and MOO3 doses of serum were added to the surface of CAM carrier. CAM specimens were prepared after 3 days of incubation. Angiogenesis was observed, And neovascular count. Results: The angiogenesis in MOO and bFGF group was better than that in NS group and blank serum group. The number of neovascularization in each MOO group was significantly higher than that in blank serum group (P <0.05), but the effect was weaker than that in bFGF group (P <0.05). Compared with MOO low dose group, neovascularization number increased significantly (P <0.05) in middle and high dose group, but there was no significant difference between the two groups. There was no significant difference in the number of neovascularization between NS group and blank serum group. CONCLUSION: MOO can promote vascularization of chick chorioallantoic membrane and have certain pro-angiogenic effect.