制备抗体的F(ab′) 2 片段的传统方法是胃蛋白酶消化法 ,然而不同IgG亚类对胃蛋白酶消化的敏感性不一样 ,尤其是IgGl最难消化 ,甚至有抗性 ,得率很低[1] 。本文报道了F(ab′) 2 片段制备的一种新方法—木瓜蛋白酶法。我们采用该方法将抗人肝癌抗体HAb18、抗人SIgA单抗 4E3 酶切 The traditional method for preparing F (ab ’) 2 fragments of antibodies is pepsin digestion, however, the sensitivity of different IgG subtypes to pepsin digestion is different, especially IgG1 is the most difficult to digest and even resistant, with a very low yield [1] . This paper reports a new method for the preparation of F (ab ’) 2 fragments - papain. We use this method to anti-human liver cancer antibody HAb18, anti-human SIgA monoclonal antibody 4E3 digestion