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目的 :探讨肠复康合剂对裸鼠移植性人结肠癌 HT- 2 9血管生成的机制。方法 :将 2 4只裸小鼠随机分为模型组、肠复康组及西药组 ,每组 8只。各组分别于建立人结肠癌 HT- 2 9癌细胞株裸小鼠皮下移植瘤模型后 6 d给予相应药物 ,免疫组化染色结合图像分析系统半定量检测移植瘤组织微血管密度 (MVD)、血管内皮生长因子(VEGF)及其受体 (FL K- 1)、内皮抑素 (ES)的积分光密度 (IOD) ,计算 VEGF/ES比值 ,并使用逐步引入剔出模型进行单因素和多因素线性回归分析。结果 :与模型组相比 ,肠复康组可使移植瘤 MVD、VEGF、FL K- 1及 VEGF/ES比值显著降低 (P <0 .0 1) ,同时使 ES含量明显增高 (P <0 .0 1) ;西药组可明显降低瘤组织 VEGF、FL K- 1含量 (P<0 .0 1) ,但不能使移植瘤 MVD、ES和 VEGF/ES比值减少 (P >0 .0 5 )。单因素回归分析显示 ,瘤组织 VEGF、ES和 VEGF/ES比值与移植瘤 MVD相关 ,多因素回归分析显示 ,移植瘤 MVD与 VEGF/ES比值呈明显正相关 (r=0 .76 2 ,P <0 .0 1)。结论 :肠复康合剂能抑制人结肠癌 HT- 2 9裸鼠移植瘤血管生成 ,其机制可能是降低瘤组织VEGF/ES比值。
Objective: To investigate the mechanism of Changfukang Mixture on angiogenesis of transplanted human colon cancer HT-29 in nude mice. Methods: Twenty-four nude mice were randomly divided into model group, Changfukang group and western medicine group, with 8 rats in each group. Each group was given the corresponding drug 6 days after establishment of the subcutaneous xenograft tumor model of human colon cancer HT-2 9 cancer cell line. The expression of MVD and MVD were detected semi-quantitatively by immunohistochemistry and image analysis system. The integrated optical density (IOD) of VEGF, its receptor (FL K-1) and endostatin (ES) was calculated and the VEGF / ES ratio was calculated. Single- and multi-factor Linear regression analysis. Results: Compared with the model group, the intestinal Rehabilitation group could significantly reduce the MVD, VEGF, FL K-1 and VEGF / ES ratio (P <0.01), at the same time, the content of ES was significantly increased (P <0 .0 1). Western medicine group could significantly reduce the content of VEGF and FL K-1 (P <0.01), but could not decrease the ratio of MVD, ES and VEGF / ES (P> 0.05) . Univariate regression analysis showed that the ratio of VEGF, ES and VEGF / ES in tumor tissue was correlated with the MVD of tumor. Multivariate regression analysis showed that MVD was positively correlated with VEGF / ES (r = 0.762, P < 0 .0 1). CONCLUSION: Changfukang Mixture can inhibit angiogenesis in human colon cancer HT-2 xenografts in nude mice, and its mechanism may be to decrease the ratio of VEGF / ES in tumor tissue.