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目的:观察单侧输尿管梗阻(UUO)大鼠肾组织Ⅰ、Ⅲ型胶原含量,探讨黄芪丹参药对及其有效成分对肾纤维化的影响。方法:66只SD大鼠随机分为7组,正常组、模型组、福辛普利组(用蒙诺化学名取代商品名)、丹参总酚酸组、黄芪总皂苷组、黄芪丹参颗粒剂配伍1∶1组(简称颗粒配伍组)、黄芪丹参组分配伍1∶1组(简称组分配伍组)。各组手术后第15天取出梗阻侧肾脏,组织经4%多聚甲醛固定24h,然后常规脱水包埋制成石蜡切片。部分进行HE、Masson病理染色,光学显微镜下观察;另一部分进行免疫组化实验,观察Ⅰ、Ⅲ型胶原沉积情况。结果:各组肾脏病理损害、胶原沉积及免疫组化结果表现一致,均以模型组最明显;表达最轻的为福辛普利组及组分配伍组。结论:UUO大鼠肾组织胶原沉积明显,黄芪丹参药对及其有效成分干预后在一定程度上能改善肾纤维化。
Objective: To observe the content of type Ⅰ and type Ⅲ collagen in kidney of rats with unilateral ureteral obstruction (UUO), and to explore the effect of Radix Astragali and Radix Salviae Miltiorrhizae and its effective components on renal fibrosis. Methods: Sixty-six Sprague-Dawley rats were randomly divided into seven groups: normal group, model group, fosinopril group (using Mono chemical name instead of commercial name), Salvia miltiorrhiza total acid group, Astragalus total saponin group, Compatibility 1: 1 group (referred to as particle compatibility group), Astragalus Salvia ingredients compatibility 1: 1 group (referred to as the component compatibility group). Obstruction side of the kidney was removed on the 15th day after operation in each group. The tissues were fixed with 4% paraformaldehyde for 24 hours, and then paraffin sections were obtained by routine dehydration embedding. Part of HE, Masson pathological staining, optical microscopy; the other part of the immunohistochemical experiments to observe the type Ⅰ, Ⅲ collagen deposition. Results: The results of renal pathological damage, collagen deposition and immunohistochemistry were the same in all groups, which were the most obvious in model group. The lightest ones were fosinopril group and component compatibility group. Conclusion: The collagen deposition in renal tissue of UUO rats is obvious. The intervention of Astragalus and Radix Salviae Miltiorrhizae and its active ingredients can improve renal fibrosis to a certain extent.