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作者用反相HPLC 的梯度洗脱法,可同时测定复方片剂中巯甲丙脯酸(Ⅰ)与双氢氯噻嗪(Ⅱ)二种成分及其分解产物——巯甲丙腩酸二硫化物和双氢氯噻嗪二磺酰氨的含量。样品以甲醇提取,离心,将提取液注入苯基柱中,用甲醇磷酸水溶液作梯度洗脱,于210nm 波长处检测。仪器为:HPLC 梯度系统带有流速及流动相组分控制装置,连接50×0.2cm 饱和柱内装37μm 硅酸。入口滤过器(2 μm);环状进样器(20 μl);反相苯基柱,5或10μm,250～300mm×内径4.6mm;可变波长检出器210 nm 波长;柱温保持约30℃、双通道具有输入、输出功能的记录器,一通道记录(Ⅰ)及(Ⅱ)峰,
The authors used reversed-phase HPLC gradient elution method, simultaneous determination of two components of captopril (Ⅰ) and hydrochlorothiazide (Ⅱ) and its decomposition products - captopril bicarb disulfide and Dihydrochlorothiazine disulfonamide content. The sample was extracted with methanol and centrifuged. The extract was injected into a phenyl column and eluted with a methanol phosphoric acid aqueous solution at a wavelength of 210 nm. Instrument: HPLC gradient system with a flow rate and mobile phase component control device, connected 50 × 0.2cm saturated column 37μm silicate. Inlet filter (2 μm); Annular injector (20 μl); Inverted phenyl column, 5 or 10 μm, 250-300 mm × 4.6 mm ID; wavelength detector with 210 nm wavelength; About 30 ℃, dual-channel recorder with input and output functions, a channel record (Ⅰ) and (Ⅱ) peak,