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本文选取海洋桡足类日本虎斑猛水蚤(Tigriopus japonicus)为模式生物,采用生化测定和实验生态学方法,研究不同酸化胁迫对桡足类体内抗氧化性指标[超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽硫转移酶(GST)和脂质过氧化(LPO)]、幼体发育时间、世代发育时间和产后代数的影响。结果表明:海水酸化可显著诱导桡足类的抗氧化性酶(SOD和CAT)的活性,而GST活性不受酸化影响,同时酸化可显著提高桡足类的LPO水平,可见该桡足类可通过SOD和CAT活性的提高来抵抗酸化胁迫引起的氧化胁迫;另外,酸化显著缩短桡足类的幼体发育时间和世代发育时间,并显著抑制其产后代数,提示酸化可以影响海洋桡足类的种群补充和动态;酸化引起该桡足类氧化胁迫的出现,继而对细胞生物大分子(如蛋白质、核酸等)造成氧化损伤,最终影响到该动物的个体水平和种群动态(如缩短发育时间和抑制繁殖力)。
In this paper, Tigriopus japonicus, a marine copepoda, was selected as a model organism. The biochemical and experimental ecological methods were used to study the effects of different acidification stress on the antioxidant activity of superoxide dismutase (SOD) [superoxide dismutase (SOD) Catalase (CAT), glutathione S-transferase (GST) and lipid peroxidation (LPO)], larval development time, generational developmental time and postpartum algebra. The results showed that seawater acidification significantly induced the activities of antioxidant enzymes (SOD and CAT) of copepods, while the activities of GST were not affected by acidification. At the same time, acidification significantly increased the LPO levels of copepods. In addition, acidification significantly reduced larval development time and generational development time of copepods and significantly inhibited postnatal algebra, suggesting that acidification may affect the population of marine copepods Supplementation and dynamics; acidification causes the emergence of oxidative stress in the copepod, which in turn causes oxidative damage to cellular biological macromolecules (eg, proteins, nucleic acids, etc.) that ultimately affect the animal’s individual level and population dynamics (eg, shortened developmental time and inhibition Fertility).