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来自30例连续死产或流产的一个或多个组织的活组织检查,用于一项试验性研究,以测试原位系统的功效。组织采用胎盘、羊膜、皮肤、脐带、肌肉和/或性腺。方法为:组织经胶原酶解聚后,接种到盖玻片上,在低氧压条件下开放系统培养,收获前在溴脱氧尿核苷(BrdU)和乙酰甲基秋水仙碱中过夜孵育,用自动收获仪原位收获。染色体经波长254um的紫外灯(UV)照射40s后,G显带用常规的胰酶处理方法,变化是玻片加热至60℃,在10％过氧化氢中处理40s。细胞的中期分裂相质量用UK NEQAS评分系统测定:单套染色体(bphs)的G带为150条带时,记2分,表示“差”;400bphs时记4分,表
Biopsies from one or more of 30 consecutive stillbirths or abortions were used in a pilot study to test the efficacy of the orthotopic system. Tissues employ placenta, amniotic membrane, skin, umbilical cord, muscle and / or gonads. The method is as follows: the tissue is depolymerized by collagenase, inoculated into a cover glass, cultured in an open oxygen system at low oxygen pressure, incubated overnight in bromodeoxyuridine (BrdU) and acetylsalicylic colchicine, and incubated with Automatic harvester harvest in situ. Chromosomes were irradiated with ultraviolet light (UV) of wavelength 254um for 40s. G banding was performed by conventional trypsin treatment. The changes were that the slide was heated to 60 ° C and treated with 10% hydrogen peroxide for 40s. The quality of metaphase cells was measured by the UK NEQAS scoring system: when the G band of a single set of chromosomes (bphs) was 150 bands, 2 points were recorded, indicating “poor”; 4 points at 400 bphs,