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目的 :探讨在不同氧浓度下吸入不同浓度NO治疗胎粪吸入综合征大耳白兔 ,对兔肺中的中性粒细胞表面粘附分子CD11b表达的影响。方法 :对常频通气下的胎粪吸入综合征家兔分别在 2 1% ,10 0 %氧浓度下给予0 2× 10 -6 mol/L ,0 3 3× 10 -6 mol/L ,0 67× 10 -6 mol/LNO治疗 12h ,通过左颈总动脉插管检测平均动脉压 ,血液高铁血红蛋白含量的变化。并采用流式细胞术检测肺泡灌洗液中中性粒细胞表面粘附分子CD11b的平均荧光强度。结果 :各组动物在各时间点平均动脉血压无差别。在两种氧浓度不同NO吸入浓度下血液各时点高铁血红蛋白含量在正常范围之内。不同氧浓度下 ,0 3 3× 10 -6 ,0 67× 10 -6 mol/LNO吸入治疗组肺泡灌洗液中的中性粒细胞上粘附分子CD11b的表达比非干预组要低 (平均数±标准差 :2 1%O2 ,0 3 3× 10 -6 mol/LNO ,12 1± 2 0vs 3 92± 2 0 4,0 67×10 -6 mol/LNO ,112± 3 0vs 3 92± 2 0 4;10 0 %O2 ,0 3 3× 10 -6 mol/LNO ,113± 2 4vs 2 93± 65 ,0 67× 10 -6 mol/LNO ,10 2±114vs 2 93± 65 ,P <0 0 5 ) ;0 2× 10 -6 mol/LNO吸入治疗组对粘附分子的表达无影响。 (平均数±标准差 :2 1%O2 ,190± 10 1vs 3 92± 2 0 4;10 0 %O2 ,2 2 2± 85vs 2 93± 65 ;P >0 0 5 ) ;同?
OBJECTIVE: To investigate the effects of different concentrations of NO on the expression of CD11b on the neutrophils in rabbits lungs after inhaling different concentrations of NO in rabbits with meconium aspiration syndrome. Methods: The rabbits with meconium aspiration syndrome under normal frequency ventilation were treated with 0 2 × 10 -6 mol / L, 0 3 3 × 10 -6 mol / L and 0 67 × 10 -6 mol / LNO for 12 hours. The changes of mean arterial pressure and blood methemoglobin in the left common carotid arteries were measured. The mean fluorescence intensity of neutrophil adhesion molecule CD11b in bronchoalveolar lavage fluid was detected by flow cytometry. Results: The average arterial blood pressure of each group of animals was not different at all time points. The concentrations of methemoglobin in blood at different concentrations of NO at two different concentrations were within the normal range. At different oxygen concentrations, the expression of CD11b on the neutrophils in bronchoalveolar lavage fluid of 0 3 3 × 10 -6 and 0 67 × 10 -6 mol / L LNO inhalation groups was lower than that of non-intervention group (mean ± standard deviation: 2 1% O2, 0 3 3 × 10 -6 mol / LNO, 12 1 ± 2 0 vs 3 92 ± 2 0 4, 0 67 × 10 -6 mol / LNO, 112 ± 3 0 vs 3 92 ± 104 ± 10 6 mol / LNO, 113 ± 2 4 vs 2 93 ± 65, 0 67 × 10 -6 mol / LNO, 10 2 ± 114 vs 2 93 ± 65, P < 0 0 5); 0 2 × 10 -6 mol / LNO inhalation treatment group had no effect on the expression of adhesion molecules. (Mean ± standard deviation: 21% O2, 190 ± 10 1 vs 3 92 ± 2 0 4; 100% O2, 2 2 2 ± 85 vs 2 93 ± 65; P> 0 0 5)