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从梗型光敏核不育材料农垦58s中提取外源DNA,在水稻幼穗发育2～4期,大量直接注射到受体亲本晚40和南京11中,成功地诱导出大批遗传变异材料,并获得了几个育性具有光敏转换特性的不育系。酯酶同工酶及过氧化物酶同工酶分析表明,六个变异株系的两种同工酶酶谱与受体存在较大差异,且具有供体农垦58s的特征酶带。同工酶变异的程度可以与形态特征的差异程度相互印证。
The exogenous DNA was extracted from the 58s of Nongken 581, a photoperiod-sensitive photoperiod-sensitive plant, and a large number of direct inoculation of late-40 and Nanjing-11 recipients of rice panicles were induced in early stage of rice panicle. Large numbers of genetic variation materials were successfully induced Several sterile lines with photoconversion characteristics were obtained. Esterase isoenzyme and peroxidase isozymes analysis showed that the two isozymes of the two isozymes showed significant differences from the receptor and had the characteristic enzyme bands of the donor Nongken 58s. The degree of isozyme variation and morphological characteristics of the degree of mutual proof.