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目的:筛选女性重度抑郁障碍患者与健康女性之间呈现差异表达的环状RNA(circRNA)，探索可能与抑郁障碍相关的目标circRNA。方法:采用高通量测序筛选3例女性重度抑郁障碍患者差异表达的circRNA，通过|logn 2FC|≥1, FDR< 0.05判定circRNA是否具有表达差异。选择差异程度最大的5个circRNA，根据circRNA的miRNA海绵功能，利用在线数据库预测circRNA可能靶向的miRNA，而后预测miRNA相关靶基因。通过GO生物学过程富集分析和KEGG信号通路富集分析，预测靶基因相关的生物学过程和信号通路。根据高通量测序结果，且具有与抑郁障碍相关的生物学过程及信号通路为依据，筛选与抑郁障碍相关的circRNA。n 结果:通过差异程度最大的5个circRNA(hsa_circ_0020959、hsa_circ_0005959、hsa_circ_0033064、hsa_circ_0006862、hsa_circ_0027732)，预测出13个靶向的miRNA及靶基因，通过靶基因预测出了多个抑郁障碍相关的生物学过程和信号通路，如糖皮质激素受体信号通路、白介素-7的反应、神经系统发育、Wnt信号通路、FoxO信号通路、甲状腺激素信号通路、神经营养素信号通路等。结论:5个circRNA均富集出了与抑郁障碍相关的生物学过程或信号通路，其中hsa_circ_0005959、hsa_circ_0033064、hsa_circ_0006862、hsa_circ_0027732与女性重度抑郁障碍的关联可能更密切。“,”Objective:To screen the circRNAs with differential expression between female patients with major depressive disorder and healthy women, and to explore the circRNAs that might be associated with depressive disorder through bioinformatics analysis.Methods:Using high-throughput sequencing screen differentially expressed circRNAs of female major depressive disorder patients, |logn 2FC|≥1 and FDR<0.05 were used to determine whether circRNA had a difference in expression.According to the miRNA sponges function of circRNA, the online databases were used to predict miRNAs which may be targeted by circRNAs, and miRNAs related target genes were also predicted by the five most different circRNA.GO and KEGG pathway enrichment analysis were used to predict biological processes for the target genes and signaling pathways.Based on the results of high-throughput sequencing, biological processes and signaling pathways related to depression, circRNAs related to depression were screened.n Results:Thirteen targeted miRNAs were predicted by the five most different circRNAs(hsa_circ_0020959, hsa_circ_0005959, hsa_circ_0033064, hsa_circ_0006862, hsa_circ_0027732), and multiple biological processes and signaling pathways related to depressive disorders were predicted by the target genes, such as glucocorticoid receptor signaling pathway, interleukin-7 response, nervous system development, Wnt signaling pathway, FoxO signaling pathway, thyroid hormone signaling pathway, neurotrophin signaling pathway, and so on.Conclusion:All the 5 circRNAs enriched biological processes or signaling pathways related to depressive disorder, among which hsa_circ_0005959, hsa_circ_0033064, hsa_circ_0006862 and hsa_circ_0027732 may be more closely related to female major depressive disorder.