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目的 分析甲肝病毒基因型,为确定我国甲肝的分子流行病学及疫苗的安全性和免疫原性奠定基础。方法 采用抗原捕获聚合酶链反应（AC/PCR）,依据国际上甲肝病毒分型标准,扩增VP1/2A结合处的168个核苦酸（nt2909～3264）。结果 经生物信息学软件分析,得出L-A-1株病毒属于甲肝病毒基因IB亚型。结论 在我国有IA和IB两个亚型甲肝病毒流行。
Objective To analyze the genotype of hepatitis A virus and lay the foundation for the molecular epidemiology of hepatitis A and the safety and immunogenicity of the vaccine. Methods Antigen - capture polymerase chain reaction (AC / PCR) was used to amplify 168 nucleotides (nt2909 ~ 3264) of VP1 / 2A junction according to the international standard of hepatitis A virus classification. Results The results of bioinformatics software analysis showed that L-A-1 strain belongs to the hepatitis B virus genotype IB subtype. Conclusions There are two subtypes of hepatitis A virus epidemic in China.