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目的 :研究富血小板血浆(platelet rich plasma,PRP)对负载人胚胎来源的间充质干细胞(h ESC-MSCs)多孔磷酸钙骨水泥(calcium phosphate cements,CPC)复合物的成骨促进作用。方法:将h ESC-MSCs接种于CPC表面,扫描电镜(SEM)观察h ESC-MSCs在CPC表面的黏附情况。建立直径为8 mm的大鼠颅骨圆形缺损模型,将18只大鼠随机分为3组,分别是CPC+h ESC-MSCs组、CPC+h ESC-MSCs+30%PRP组和CPC+h ESC-MSCs+50%PRP组。术后12周显微CT和H-E染色观察新骨和血管生成情况。应用SPSS16.0软件包对数据进行单因素方差分析。结果:SEM显示,细胞可良好地黏附在CPC支架的表面。显微CT示复合PRP后,CPC材料的降解程度逐渐明显,且新生骨量逐渐增加。H-E染色显示,复合PRP组的新骨生成率和血管密度高于未复合PRP组,但是复合不同浓度的PRP组间无显著差异。结论:浓度为30%和50%的PRP与负载h ESC-MSCs的CPC复合后,均可促进骨缺损区新骨的生成。
OBJECTIVE: To study the osteogenesis promoting effect of platelet rich plasma (PRP) on calcium phosphate cements (CPC) complexes loaded human embryonic mesenchymal stem cells (h ESC-MSCs). METHODS: h ESC-MSCs were inoculated on CPC surface and the adhesion of h ESC-MSCs on CPC surface was observed by scanning electron microscope (SEM). The model of 8-mm diameter rat skull defects was established. Eighteen rats were randomly divided into three groups: CPC + h ESC-MSCs group, CPC + h ESC-MSCs + 30% PRP group and CPC + h ESC-MSCs + 50% PRP group. At 12 weeks after operation, micro-CT and H-E staining were used to observe the new bone and angiogenesis. One-way ANOVA was performed using SPSS16.0 software package. Results: SEM showed that cells adhered well to the surface of CPC scaffolds. Micro-CT showed PRP compound, CPC degradation of the material gradually obvious, and the amount of new bone gradually increased. H-E staining showed that the new bone formation rate and vascular density in the composite PRP group were higher than those in the uncompounded PRP group, but there was no significant difference between the composite PRP group and the PRP group. CONCLUSION: The combination of 30% and 50% PRP with CPC loaded with h ESC-MSCs can promote the formation of new bone in the bone defect area.