利用苯酚对血红蛋白模拟酶催化H2O2和L-酪氨酸荧光体系的猝灭作用,建立了酶催化荧光法测定痕量苯酚的新方法。研究了该猝灭反应的最佳实验条件,其猝灭程度与苯酚的浓度成线性关系。测定的线性范围为3.0~70μmol/L,检出限为1.64μmol/L。对浓度为15μmol/L的苯酚标准溶液进行了11次平行测定,其相对标准偏差为3.0%。方法可用于环境水样中苯酚含量的测定。 A new method for the determination of trace phenol by enzymatic fluorimetry was established by using phenol to quench the fluorescence of H2O2 and L-tyrosine catalyzed by hemoglobin mimic enzyme. The best experimental conditions of the quenching reaction were studied. The quenching degree of the quenching reaction was linear with the concentration of phenol. The linear range was 3.0 ~ 70μmol / L, the detection limit was 1.64μmol / L. Eleven parallel determinations of phenol standard solutions at a concentration of 15 μmol / L showed a relative standard deviation of 3.0%. The method can be used for the determination of phenol content in environmental water samples.