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目的:通过研究儿童Ⅰ型糖尿病患者中转录因子FOXP3的表达,探讨其在儿童Ⅰ型糖尿病发病的临床意义。方法:选择患儿与正常人各30例,用电化学发光法检测血清中C肽的含量;全自动生化分析仪检测空腹血糖;流式细胞仪检测外周血CD4+CD25+调节性T细胞(regulatory T cell,Treg)的数量;实时定量PCR(RT-PCR)检测FOXP3的mRNA表达。结果:Ⅰ型糖尿病患儿组中血清C肽含量(0.71±0.06)ng/ml明显低于正常对照组(2.45±0.28)ng/ml;血糖含量(7.51±5.06)mmol/L明显高于正常对照组(4.85±0.68)mmol/L;外周血中CD4+CD25+Treg约占CD4+T细胞(1.57±0.15)%,低于正常对照组(3.13±0.29)%(P<0.05);Ⅰ型糖尿病患儿组外周血CD4+CD25+Treg细胞低表达FOXP3。结论:儿童Ⅰ型糖尿病患者外周血FOXP3表达降低,导致机体不能抑制效应性CD4+T细胞的增殖对胰岛的破坏,从而参与了Ⅰ型糖尿病的发生。
Objective: To investigate the clinical significance of FOXP3 expression in children with type 1 diabetes mellitus (T2DM) by studying the expression of FOXP3 in children with type 1 diabetes mellitus. Methods: 30 cases of children and normal subjects were selected. The content of C-peptide in serum was detected by electrochemiluminescence. Fasting blood glucose was detected by automatic biochemical analyzer. The levels of CD4 + CD25 + regulatory T cells in peripheral blood were detected by flow cytometry T cell and Treg). The mRNA expression of FOXP3 was detected by real-time quantitative PCR (RT-PCR). Results: Serum C-peptide levels in children with type 1 diabetes (0.71 ± 0.06) ng / ml were significantly lower than those in normal controls (2.45 ± 0.28) ng / ml; blood glucose levels (7.51 ± 5.06) mmol / L were significantly higher than those in normal controls The percentage of CD4 + CD25 + Treg in peripheral blood was (1.57 ± 0.15)% of that in control group (4.85 ± 0.68) mmol / L, which was lower than that of control group (3.13 ± 0.29)% The expression of FOXP3 in peripheral CD4 + CD25 + Treg cells in children with type. Conclusion: The expression of FOXP3 in children with type 1 diabetes mellitus is decreased, leading to the inability of the body to inhibit the destruction of islet by the proliferation of effector CD4 + T cells, which is involved in the development of type 1 diabetes.