为了解决天然水蛭素来源匮乏问题，我们采用基因工程技术进行重组水蛭素的研究。根据水蛭素变异物１（ＨＶ１）的氨基酸序列，选择在原核细胞中高表达的密码子，设计了２２１个碱基对长度的ＨＶＩＤＮＡ片段。分１４个寡核苷酸片段进行化学合成，各片段连接成ＨＶ１全基因后，经ＥｃｏＲＩ、ＢａｍＨＩ切点克隆入ｐＵＣ１８／１９质粒，转化大肠杆菌ＪＭ１０５，蓝白菌落法筛选阳性克隆。限制性内切酶实验及ＤＮＡ序列分析证实了ＨＶ１全基因的正确性，克隆成功。并且获得活性表达。 In order to solve the problem of the lack of natural hirudin, we used the genetic engineering technology to study the recombinant hirudin. Based on the amino acid sequence of hirudin variant 1 (HV1), codons highly expressed in prokaryotic cells were selected and a 221 base pair length HVIDNA fragment was designed. Fourteen oligonucleotide fragments were chemically synthesized. Each fragment was ligated into the HV1 gene and cloned into pUC18 / 19 by EcoRI and BamHI cleavage points. The recombinant plasmid was transformed into E. coli JM105. The restriction endonuclease assay and DNA sequence analysis confirmed the correctness of the HV1 gene and the cloning was successful. And obtain active expression.