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以氯霉素(CAP)为模板分子、甲基丙烯酸N,N-二乙基氨基乙酯(DEAEM)为功能单体、二甲基丙烯酸乙二醇酯(EGDMA)为交联剂、聚苯乙烯微球作为种球,采用两步溶胀聚合的方法制备得到了粒径均一的分子印迹聚合物微球(MIPMs)。结果表明:扫描电镜观察显示其表面为多孔结构;通过孔容和孔径研究表明,与非印迹聚合物微球(NIPMs)相比,MIPMs具有更大的孔径和孔体积;进一步通过液相色谱对其选择性能进行评价,可知氯霉素、甲砜霉素、氟甲砜霉素在MeCN/Water/H3PO4(30:70:0.1,V/V)流动相条件下,3种物质达到了较好的分离效果,获得的MIPMs对3种物质具有较好的选择性能。
Using chloramphenicol (CAP) as template molecule, N, N-diethylaminoethyl methacrylate (DEAEM) as functional monomer, ethylene glycol dimethacrylate (EGDMA) as crosslinking agent, Ethylene microspheres were used as bulbs to prepare molecularly imprinted polymer microspheres (MIPMs) with uniform particle size by two-step swelling polymerization. The results showed that the surface was porous by scanning electron microscopy. The study of pore volume and pore size showed that MIPMs had larger pore size and pore volume than non-imprinted polymer microspheres (NIPMs) The selectivity of chloramphenicol, thiamphenicol and florfenicol reached the best under the mobile phase of MeCN / Water / H3PO4 (30: 70: 0.1, V / V) The separation effect of MIPMs obtained on the three substances have better selectivity.