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为探讨甘草炮制雷公藤降低其肝毒性的作用,动物实验评价炮制前后雷公藤对肝脏的毒性,观察空白组、雷公藤组、甘草炮制雷公藤组肝组织病理切片、血清生化指标及细胞炎症因子的差异。大鼠肝组织病理切片显示雷公藤组肝组织损伤明显,炮制后肝组织未见明显损伤;雷公藤组与空白组比较AST,ALT,CRE升高(P<0.01),UREA升高(P<0.05),ALB降低(P<0.01);甘草炮制雷公藤组与雷公藤组比较AST,ALT,CRE,UREA降低(P<0.01),ALB升高(P<0.01)。炎症因子检测结果显示雷公藤组与空白组比较IL-1β,IL-6,TNF-α显著升高(P<0.01);甘草炮制雷公藤组与雷公藤组比较IL-1β,IL-6,TNF-α显著降低(P<0.01)。甘草炮制雷公藤可有效降低雷公藤肝毒性,减轻雷公藤导致的肝损伤。该实验可为雷公藤合理制用提供参考,同时为甘草炮制雷公藤降低其肝毒性研究提供数据支持。
In order to explore the effect of licorice processed Tripterygium on reducing its hepatotoxicity, the toxicity of Tripterygium wilfordii to liver was evaluated in animal experiments. The pathological sections of liver tissue, serum biochemical indexes and cytokines were observed in blank group, tripterygium wilfordii, The difference. The pathological sections of rat liver showed that the injury of liver tissue of Tripterygium wilfordii was obvious, and no significant damage was observed in the liver tissue after processing. The levels of AST, ALT and CRE increased (P <0.01) and UREA increased in tripterygium wilfordii group compared with the blank group (P < (P <0.01). The levels of AST, ALT, CRE and UREA in the tripterygium wilfordii group were significantly lower than those in the tripterygium wilfordii group (P <0.01). The results of inflammatory cytokines showed that IL-1β, IL-6 and TNF-αwere significantly increased in tripterygium wilfordii group compared with the blank group (P <0.01) TNF-αdecreased significantly (P <0.01). Licorice processing Tripterygium can effectively reduce the toxicity of Tripterygium wilfordii Reduce Tripterygium caused by liver damage. The experiment can provide a reference for the rational use of Tripterygium wilfordii, and provide data support for the study of Tripterygium wilfordii to reduce its hepatotoxicity.