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目的:为更好模拟儿科肠外营养相关并发症，拟用新生巴马小猪建立肠内及肠外营养相关模型。方法:新生巴马小猪18只，分为肠内营养(enteral nutrition, EN)组和全肠外营养(total parenteral nutrition, TPN)组。EN组包括喂养1周(EN-1W, n n＝3)和喂养2周(EN-2W, n n＝3)小猪；TPN组包括肠外营养支持1周(TPN-1W, n n＝6)和肠外营养支持2周(TPN-2W, n n＝6)小猪。观察记录小猪体重和组织形态学；检测分析肝功能和肠屏障功能。n 结果:造模期间TPN小猪全部存活，TPN-2W小猪肠外营养相关并发症较TPN-1W更明显。与EN-2W组对照相比，TPN-2W小猪的体重下降明显，差异有统计学意义(n P<0.05)。TPN-2W小猪血清中的总胆红素、直接胆红素及胆汁酸含量明显高于EN-2W组，差异均有统计学意义(n P<0.05)。肝脏形态学显示，TPN小猪肝脏门脉区有炎症细胞浸润并伴有肝细胞轻度脂肪变。与EN-2W组对照相比，TPN-2W小猪回肠绒毛萎缩变形、绒毛长度明显变短(n P<0.05)、隐窝深度变浅(n P<0.01)，其肠通透性明显增加(n P<0.05)。n 结论:建立新生巴马小猪的TPN模型，模拟了婴幼儿肠外营养相关肝损害及肠屏障损伤等并发症，为研究儿科肠外营养相关并发症的发病机制提供了较好的动物模型。“,”Objective:To better simulate parenteral nutrition-associated complications in pediatrics, establishment of enteral and parenteral nutrition-related models were planned with newborn Bama mini-pig.Methods:Eighteen newborn Bama mini-pigs were divided into enteral nutrition (EN) groups and total parenteral nutrition (TPN) groups. EN groups were further divided into EN-1W (n n=3) and EN-2W (n n=3); and TPN groups were divided into TPN-1W (n n=6) and TPN-2W (n n=6). The weight and histomorphological changes of newborn Bama mini-pigs were observed. Liver function and intestinal barrier function were also detected and analyzed.n Results:During modeling, all Bama mini-pigs in TPN groups survived, the related complications of TPN-2W mini-pigs were more obvious than that of TPN-1W mini-pigs. Compared with EN-2W group, the weight of TPN-2W group decreased significantly (n P<0.05). Serum total bilirubin, direct bilirubin, and bile acid levels in TPN-2W group were significantly higher than those in EN-2W group (n P<0.05). The hepatic histomorphology of the TPN groups showed scattered portal inflammatory infiltration and mild hepatic steatosis. Compared with EN-2W group, the intestinal villi of the TPN-2W mini-pigs were atrophic and deformed, with significantly decreased villus length (n P<0.05), and crypt depth (n P<0.01), and significantly increased intestinal permeability (n P<0.05).n Conclusion:An experimental model of total parenteral nutrition can be established using the newborn Bama mini-pigs, which can well simulate the parenteral nutrition-associated liver damage and intestinal barrier injury in infants and young children, thus providing a better animal model to study the pathogenesis of clinical parenteral nutrition-associated complications in pediatrics.