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目的 :研究 4 1BBL在几种人肿瘤细胞株上的表达。方法 :用RT PCR法检测肿瘤细胞内 4 1BBLmRNA水平的表达 ;用流式细胞仪检测肿瘤细胞膜表面 4 1BBL的表达。结果 :4 1BBL在Jurkat细胞株上高表达 (98.97% ) ,在HL 6 0和K5 6 2上表达较低 (分别为 2 4 .77%和 19.2 3% ) ,而在HT 2 9细胞株上基本不表达 (2 .13% )。结论 :4 1BBL在不同肿瘤细胞株表达水平不同
Objective: To investigate the expression of 4 1BBL in several human tumor cell lines. Methods: RT-PCR was used to detect the level of 4 1BBL mRNA in tumor cells. The expression of 4 1BBL on tumor cell membrane was detected by flow cytometry. RESULTS: 4 1BBL was highly expressed on Jurkat cells (98.97%), lower on HL 60 and K562 (24.77% and 19.23%, respectively), whereas on HT2 9 cells Basically not expressed (2.13%). Conclusion: 4 1BBL expression levels in different tumor cell lines are different