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目的探讨白细胞介素1β(IL-1β)与地塞米松联合应用对人成骨细胞(HumanOsteoblast-likecellsHOS)芳香化酶活性的影响。方法对人的成骨细胞进行培养,设立实验组,分别加入IL-1β1ng/mL(实验Ⅰ组)、10ng/mL(实验Ⅱ组)、50ng/mL(实验Ⅲ组);分别加地塞米松浓度0.1uM(实验Ⅳ组)、1.0uM(实验Ⅴ组)、10.0uM(实验Ⅵ组);地塞米松浓度0.1uM和IL-1β1ng/mL(实验Ⅶ组);IL-1β10ng/mL和IL-1ra500ng/mL(实验Ⅷ组),对照组加培养液,采用氚水法检测芳香化酶活性。结果实验组Ⅰ,Ⅱ,Ⅲ组氚水放射强度与对照组比较放射强度明显增加,差异显著(P<0.05)。实验Ⅳ,Ⅴ,Ⅵ组放射强度与对照组比较有非常显著性差异(P<0.001)。实验Ⅶ组与对照组间差异无统计学意义.实验Ⅷ组放射强度高于对照组及实验组Ⅰ和Ⅳ,差异非常显著(P<0.001)。结论人成骨细胞中IL-1β、地塞米松分别可以增加芳香化酶的活性。IL-1β是通过与成骨细胞的IL-1受体相结合而起作用。地塞米松和IL-1β联合应用使芳香化酶活性增加,它们相互作用影响着骨骼中雌激素合成。
Objective To investigate the effect of interleukin-1β (IL-1β) combined with dexamethasone on the aromatase activity of human osteoblast-like cells (COS). Methods Human osteoblasts were cultured, and the experimental group was set up, and IL-1β 1ng / mL (experimental group Ⅰ), 10ng / mL (experimental group Ⅱ) and 50ng / mL 0.1uM (experimental group IV), 1.0uM (experimental group V), 10.0uM (experimental group VI), dexamethasone 0.1uM and IL-1beta1ng / mL (experimental group VII) 1ra500ng / mL (experimental group Ⅷ), the control group plus broth, aromatase activity was measured by tritiated water method. Results The intensity of tritiated water in experimental group Ⅰ, Ⅱ and Ⅲ increased significantly compared with the control group (P <0.05). There was a significant difference (P <0.001) between the radiation intensity of groups Ⅳ, Ⅴ and Ⅵ of the experiment group and the control group. There was no significant difference between experimental group Ⅶ and control group.The radiation intensity of experimental group Ⅷ was higher than that of control group and experimental group Ⅰ and Ⅳ (P <0.001). Conclusion Human osteoblasts IL-1β and dexamethasone can increase the activity of aromatase, respectively. IL-1β acts by binding to the IL-1 receptor of osteoblasts. The combination of dexamethasone and IL-1β increases the aromatase activity and their interaction affects estrogen synthesis in the bones.