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目的采用微板核酸杂交-ELISA技术测定肝炎病人血清中HBVDNA含量。方法用PCR法将病人血清标本扩增后，与两条特异性探针夹心杂交，然后通过酶联显色对69例不同临床表现的肝炎患者血清中HBVDNA进行定量检测。结果20例急性重型肝炎血清中的DNA含量超过2μg/L，88.24％的急性轻型肝炎患者和54.55％轻度慢性肝炎患者血清中的HBVDNA含量少于2μg/L,66.67％中度慢性肝炎患者血清HBVDNA含量在2-1000μg/L的范围，75％重度慢性肝炎和45％急性重型肝炎血清中的HBVDNA含量超过1000μg／L。结论做饭核酸杂交-ELISA检测方法能准确、快速进行核酸定量测定，特异性强，灵敏度高，稳定可靠，易自动化，对于研究病毒感染量与临床病程的关系具有较大的现实意义。
Objective To determine the content of HBVDNA in the serum of hepatitis patients by using microplate nucleic acid hybridization-ELISA technique. Methods Serum samples from patients were amplified by PCR and hybridized with two specific probes. HBVDNA in sera of 69 patients with different clinical manifestations was quantitatively detected by enzyme-linked immunosorbent assay. Results The serum levels of DNA in 20 cases of acute severe hepatitis were more than 2μg / L. The serum HBVDNA in 88.24% of patients with acute hepatitis and 54.55% of mild chronic hepatitis was less than 2μg / L, 66.67% of patients with moderate chronic hepatitis HBVDNA content in the range of 2-1000μg / L, HBVDNA content in serum of 75% severe chronic hepatitis and 45% acute severe hepatitis exceeds 1000μg / L. Conclusion The nucleic acid hybridization-ELISA method can detect nucleic acid quantitatively and rapidly with high specificity, high sensitivity, stability and reliability, and is easy to be automated. It is of great practical significance to study the relationship between viral infection and clinical course.