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目的利用小鼠肝癌细胞株H22细胞肝脏原位种植法建立BALB/c小鼠原位移植肝癌模型,为研究肝脏肿瘤奠定基础。方法取新鲜小鼠腹腔传代的H22细胞,生理盐水洗涤2次,调细胞浓度为1×10~7/ml,与Matrigel按4:1比例混合,用10μl微量进样针在开腹直视下沿肝叶长轴种植于BALB/c小鼠的肝左叶,每只5μl,逐层关腹,常规饲养2周后,处死小鼠观察肿瘤形成情况,检测小鼠肝脏原住癌的形成率及小鼠存活率,形态学和病理学方法鉴定造模效果。结果肉眼可见小鼠肝脏出现单个灰白肿瘤结节,病理学方法证实为肝癌,小鼠存活率和成瘤率均为95%,肿瘤大小均匀。结论成功建立了一种稳定、简单可行的BALB/c小鼠原位移植肝癌模型。
OBJECTIVE: To establish a orthotopic liver transplantation model of BALB / c mice by in situ hepatocyte implantation using mouse hepatoma cell line H22 and lay the foundation for the study of liver tumors. Methods Freshly passaged H22 cells from the peritoneal cavity of mice were washed twice with normal saline and adjusted to a cell concentration of 1 × 10 ~ 7 / ml. The cells were mixed with Matrigel at a ratio of 4: 1. The left lobe of the liver of BALB / c mice was planted along the long axis of the liver lobe, each with 5μl layer and the stomach closed. The mice were sacrificed after 2 weeks of routine feeding to observe the tumor formation and to detect the rate of liver cancer And mouse survival rate, morphological and pathological methods to identify the modeling effect. Results The naked eye showed a single gray tumor nodules in the liver, pathologically confirmed as liver cancer, mouse survival rate and tumor formation rate was 95%, tumor size. Conclusion A stable and simple model of orthotopic liver transplantation in BALB / c mice was successfully established.