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目的观察冠心病患者血清多种细胞因子的变化及相互关系,以探索血清细胞因子谱在冠心病诊断中的应用价值。方法观察76例确诊的冠心病患者及26名健康查体者血清细胞因子水平,利用 RANDOX 细胞因子芯片和 EVIDENCE 全自动芯片分析仪测定血清12项细胞因子,运用生物信息学方法绘制血清细胞因子图谱。结果冠心病患者血清白细胞介素(IL)-1β、IL6、IL8、肿瘤坏死因子(TNF)-α水平分别为(4.32±8.14)ng/L,(13.16±16.81)ng/L,(375.53±292.14)ng/L,(15.46±15.38)ng/L,其浓度显著高于正常人群(P<0.01)。IL-4水平为(0.65±1.51)ng/L,低于正常人群(P<0.05)。稳定型心绞痛、不稳定型心绞痛和急性心肌梗死组患者血清中 IL-1β、IL-4、IL-6、IL-8、血管内皮生长因子(VEGF)、TNF-α、单核细胞趋化蛋白-1(MCP-1)、表皮生长因子(EGF)等均呈不同程度变化,特别是 IL-6、IL-8、VEGF、TNF-α水平变化幅度最大。结论血清多种细胞因子在冠心病不同分型中呈规律性变化,提示细胞因子谱在冠心病的诊断及预后具有潜在的应用价值。
Objective To observe the changes of serum cytokines in patients with coronary heart disease and their relationship to explore the value of serum cytokine profiles in the diagnosis of coronary heart disease. Methods Serum levels of cytokines in 76 patients with CHD and 26 healthy individuals were observed. Twelve serum cytokines were measured by RANDOX cytokine chip and EVIDENCE automatic chip analyzer. The serum cytokine profiles were drawn by bioinformatics . Results The levels of serum IL-1β, IL6, IL8 and TNF-α in CHD patients were (4.32 ± 8.14) ng / L, (13.16 ± 16.81) ng / L and 292.14) ng / L, (15.46 ± 15.38) ng / L respectively, which was significantly higher than that of the normal population (P <0.01). The level of IL-4 was (0.65 ± 1.51) ng / L, lower than the normal population (P <0.05). IL-1β, IL-4, IL-6, IL-8, VEGF, TNF-α, monocyte chemoattractant protein in serum of patients with stable angina, unstable angina and acute myocardial infarction (MCP-1) and epidermal growth factor (EGF) were all changed to different degrees, especially the changes of IL-6, IL-8, VEGF and TNF- Conclusion The serum cytokines in the different types of coronary heart disease showed regular changes, suggesting that cytokine profile in the diagnosis and prognosis of coronary heart disease has potential applications.