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目的探讨脂多糖刺激单核细胞源树突状细胞表达程序性死亡因子配体1(PD-L1)与p38MAPK信号通路的关系。方法体外培养树突状细胞,用p38抑制剂SB203580阻断p38MAPK通路后再用LPS刺激树突状细胞。光学显微镜观察各组细胞的形态变化;流式细胞术测定CD86和PD-L1表达的平均荧光强度;Western blot测定PD-L1蛋白表达。结果光镜下观察LPS刺激组细胞先经历梭型贴壁后逐渐恢复圆形,树突较多;SB203580和LPS共同刺激组细胞树突退化,未刺激组细胞成团悬浮,树突较多。SB203580和LPS共同刺激组细胞CD86、PD-L1较LPS刺激组的明显降低(P<0.05或P<0.01),与未刺激组的相比CD86差异无统计学意义,但PD-L1降低(P<0.05)。SB203580和LPS共同刺激组细胞PD-L1的蛋白表达量较其它两组的均明显减少(P<0.01或P<0.01)。结论 LPS通过p38MAPK信号通路调控树突状细胞表达PD-L1。
Objective To investigate the relationship between the expression of PD-L1 and p38 MAPK signal pathway in monocytes-derived dendritic cells stimulated by lipopolysaccharide. Methods Dendritic cells were cultured in vitro. The p38 MAPK pathway was blocked by p38 inhibitor SB203580 and then dendritic cells were stimulated by LPS. The morphological changes of cells in each group were observed by light microscope. The average fluorescence intensity of CD86 and PD-L1 expression was detected by flow cytometry. The expression of PD-L1 protein was detected by Western blot. Results Under the light microscope, the cells in LPS stimulation group recovered to a round shape with more dendrites than those in the LPS stimulation group. Degradation of dendrites was observed in the cells stimulated by SB203580 and LPS, and the dendrites were more suspended in the unstimulated cells. Compared with LPS-stimulated group, the expression of CD86 and PD-L1 in SB203580 and LPS groups was significantly lower than that in LPS stimulation group (P <0.05 or P <0.01), while there was no significant difference in CD86 between unstimulated group and SB203580 <0.05). Compared with the other two groups, the protein expression of PD-L1 in SB203580 and LPS co-stimulated groups were significantly decreased (P <0.01 or P <0.01). Conclusion LPS can regulate PD-L1 expression in dendritic cells through p38 MAPK pathway.