目的：建立简便分离纯化镉诱导金属硫蛋白（ＣｄＭＴ）的方法，并制备抗ＣｄＭＴ抗血清。方法：采用加长ＳｅｐｈａｄｅｘＧ７５柱层析分离纯化由ＣｄＣｌ２诱导的大鼠肝组织ＣｄＭＴ，以此为抗原免疫家兔制得抗ＣｄＭＴ抗血清。结果：纯化的ＣｄＭＴ经ＳＤＳＰＡＧＥ测定相对分子质量及Ｃｄ含量测定与标准ＣｄＭＴ一致，氨基酸组分分析也与文献报道基本一致；获得的抗血清对大鼠ＣｄＭＴ具有特异性反应。结论：本法为一种简便高效的分离纯化ＣｄＭＴ的方法，抗ＣｄＭＴ抗血清的制备可为进一步建立敏感的ＥＬＩＳＡ和免疫组化方法进行组织内ＭＴ含量测定及定位，有利于深入研究Ｃｄ中毒机理和ＭＴ生物学功能。 Objective: To establish a simple method for the isolation and purification of cadmium-induced metallothionein (CdMT) and to prepare anti-CdMT antiserum. Methods: Using extended SephadexG  75 column chromatography purified CdCl2-induced rat liver tissue Cd  MT, as antigens to immune rabbit anti-Cd  MT antiserum. Results: The purified CdMT by SDSPAGE determination of relative molecular mass and Cd content determination consistent with the standard CdMT, amino acid composition analysis was also consistent with the literature reported; obtained antiserum specificity of CdMT in rats reaction. Conclusion: This method is a simple and efficient method for the isolation and purification of CdMT. The preparation of anti-CDMT antiserum can be used to further establish sensitive ELISA and immunohistochemical methods to determine and locate the content of MT in tissues, Study Cd poisoning mechanism and MT biological function.