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目的研究胃泌素受体(CCK B受体)的mRNA在结肠癌组织和对应癌旁正常组织中的表达水平,探讨CCK B受体参与结肠癌发病的可能机制。方法采用半定量RT PCR法检测23例新鲜结肠癌及相对应的癌旁正常组织CCK B受体的表达水平。结果23例结肠癌组织CCK B受体阳性表达率为87.0%,其表达平均值为0.704±0.134,对应癌旁正常组织CCK B受体阳性表达率为60.8%,其表达平均值为0.215±0.077。CCK B受体在结肠癌组的表达水平明显高于癌旁正常组织(P<0.05)。结论CCK B受体在结肠癌组织中的表达水平较正常组织明显增高,提示CCK B受体可能参与结肠癌的发病。
Objective To study the expression of mRNA of gastrin receptor (CCK B receptor) in colorectal cancer tissue and its adjacent normal tissues and to explore the possible mechanism of CCK B receptor participating in the pathogenesis of colon cancer. Methods The expression of CCK B receptor in 23 cases of fresh colon cancer and corresponding normal tissues was detected by semi-quantitative RT-PCR. Results The positive expression rate of CCK B receptor in colon cancer tissues was 87.0% and the average expression value was 0.704 ± 0.134. The positive expression rate of CCK B receptor in normal tissues adjacent to normal tissues was 60.8% and the average value was 0.215 ± 0.077 . The expression of CCK B receptor in colon cancer was significantly higher than that in adjacent normal tissues (P <0.05). Conclusion The expression of CCK B receptor in colon cancer tissues was significantly higher than that in normal tissues, suggesting that CCK B receptor may be involved in the pathogenesis of colon cancer.