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作者利用超速离心法从人血浆分离、纯化极低密度脂蛋白(VLDL)。VLDL脱脂后经葡聚糖凝胶层析及离子交换层析获得apoCⅠ、apoCⅡ及apoCⅢ的纯品。经等电聚焦电泳、免疫电泳、免疫双扩散、酶联免疫及放射免疫鉴定,其纯度符合要求。作者等已用此纯品免疫动物获得特异的单价抗血清,在国内首次建立了人血清载脂蛋白CⅠ、CⅡ及CⅢ的免疫测定法。
The authors isolated and purified very low density lipoprotein (VLDL) from human plasma using ultracentrifugation. VLDL defatted by dextran gel chromatography and ion exchange chromatography to obtain apoC Ⅰ, apoC Ⅱ and apoC Ⅲ pure. The isoelectric focusing electrophoresis, immunoelectrophoresis, double immunodiffusion, enzyme-linked immunosorbent assay and radioimmunoassay, its purity to meet the requirements. The authors have used this pure animal immunization to obtain specific monovalent antiserum, the first time in China to establish a human serum apolipoprotein C Ⅰ, C Ⅱ and C Ⅲ immunoassay.