等电聚焦电泳是一种有高分辨率的分析技术。用1mm厚度的聚丙烯酰胺凝胶做支持物,通常每一加样片的加样量为15μl,根据染色敏感性,蛋白浓度一般为1～15mg/ml。用考马斯亮蓝染色的最适蛋白量是每一轨道20μg。低于此量难以得到理想结果。在实际应用中有时会遇到蛋白浓度较 Isoelectric focusing electrophoresis is a high-resolution analytical technique. A 1 mm thick polyacrylamide gel is used as a support. Usually, the amount of each sample is 15 μl. The protein concentration is usually 1-15 mg / ml according to the staining sensitivity. The optimal amount of protein stained with Coomassie brilliant blue is 20 μg per track. Below this amount is difficult to get the desired result. In practical applications sometimes encounter protein concentration