论文部分内容阅读
目的:制备氯霉素(CAP)-牛血清白蛋白(BSA)及氯霉素(CAP)-卵清白蛋白(OVA)的偶联物并进行鉴定。方法:分别采用混合酸酐法和重氮化法制备CAP-BSA及CAP-OVA的偶联物,前者是将CAP先与琥珀酸酐反应生成CAP-半琥珀酸酯(CAP-HS),再进行混合酸酐反应。将反应产物CAP-HS与BSA结合,合成CAP-HS-BSA偶联物;后者是将CAP分子中的苯环上的硝基还原为氨基后,进行重氮化处理,然后分别与BSA和OVA连接,合成CAP-BSA和CAP-OVA复合物。结果:紫外图谱分析表明,CAP-BSA、CAP-OVA和CAP-HS-BSA的紫外最大吸收峰分别是262nm、213nm和275nm;计算得CAP与载体蛋白BSA、OVA及HS的偶联比分别为5∶1、12∶1和22∶1。结论:成功的制备CAP-BSA及CAP-OVA的偶联物,为免疫动物制备抗CAP的抗体奠定了基础。
Objective: To prepare and identify the conjugates of chloramphenicol (CAP) - bovine serum albumin (BSA) and chloramphenicol (CAP) - ovalbumin (OVA). Methods: The CAP-BSA and CAP-OVA conjugates were prepared by mixed anhydride method and diazotization. The former was to react CAP with succinic anhydride to form CAP-hemisuccinate (CAP-HS) Anhydride reaction. CAP-HS and BSA were combined to synthesize CAP-HS-BSA conjugate. The latter was to reduce the nitro group on the benzene ring in the CAP molecule to amino group and then diazotized, and then reacted with BSA and OVA link to synthesize CAP-BSA and CAP-OVA complexes. Results: The UV spectra of CAP-BSA, CAP-OVA and CAP-HS-BSA were 262nm, 213nm and 275nm respectively. The coupling ratios of CAP to BSA, OVA and HS were 5: 1, 12: 1 and 22: 1. Conclusion: The successful preparation of conjugates of CAP-BSA and CAP-OVA laid the foundation for the preparation of anti-CAP antibody in immunized animals.