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目的：研究肺癌组织中ｐ５３ 和 Ｋｒａｓ 基因突变、蛋白表达，以及二者的关系． 方法：应用 Ｐ Ｃ Ｒ Ｓ Ｓ Ｃ Ｐ和免疫组织化学方法分别检测肺癌组织中的ｐ５３ 和 Ｋｒａｓ基因突变和蛋白表达． 结果：肺癌组织中检测到ｐ５３ 和 Ｋｒａｓ基因的突变分别为５３％ 和１４％ ，肺癌组织中检测到ｐ５３ 蛋白和 ｐｒａｓ ２１ 蛋白的积聚分别为５８％ 和１９％ ． 结论：ｐ５３ 和 Ｋｒａｓ基因突变与肺癌发生关系密切，其基因突变与蛋白的积聚之间存在一致的关系，但并非完全对应． ｐ５３ 和 Ｋｒａｓ基因突变及其表达产物的检测对肺癌患者有一定的辅助诊断意义．
Objective: To study the mutation and protein expression of p53 and Kras genes in lung cancer tissues and their relationship. METHODS: The p53 and K-ras mutations and protein expressions in lung cancer tissues were detected by P C R S S C P and immunohistochemical methods, respectively. RESULTS: The mutations in p53 and K-ras genes were detected in lung cancer tissues at 53% and 14%, respectively. The accumulation of p53 protein and pras 21 protein in lung cancer tissues was 58% and 19%, respectively. Conclusion: The p53 and Kras gene mutations are closely related to the occurrence of lung cancer. There is a consistent relationship between gene mutations and protein accumulation, but it is not exactly corresponding. Detection of mutations in p53 and K-ras genes and their expression products have a certain significance for the diagnosis of lung cancer.