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1 资料和方法1．1 病例来源:自1993年~1998年以急性细菌性痢疾收入院,并做大便培养和做药敏试验112例现分析如下。1．2 实验方法:(1)菌株鉴定方法,取新鲜大便标本接种于SS及HE琼脂培养基,经37℃ 24小时培养后,挑选可疑菌落接种于双糖与尿素半固体培养基,如初步反应符合做血清玻片凝集,阳性者再进一步做生化反应及血清凝集定型;(2)药敏试验:采用卫生部药品生物制品检定所提供的KIRBYBUAER纸片扩散法,以抑菌区直径为判断标准。
1 Materials and Methods 1.1 Case Sources: From 1993 to 1998 with acute bacterial dysentery income hospital, and do stool training and drug sensitivity test 112 cases are as follows. 1.2 Experimental methods: (1) Strain identification methods, fresh stool specimens were inoculated on SS and HE agar medium, after 37 ° C for 24 hours after the selection of suspicious colonies were inoculated in double sugar and urea semisolid medium, such as the initial Response consistent with serum glass agglutination, positive and then do further biochemical reactions and serum agglutination stereotypes; (2) drug susceptibility test: the Ministry of Health Pharmaceutical and Biological Products provided by KIRBYBUAER disc diffusion method to determine the diameter of the bacteriostatic zone standard.