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目的:建立骨伤跌打止痛胶囊中血竭素的含量测定方法。方法:采用HPLC法,色谱柱为Shimadzu VP-ODS C18(250mm×4.6 mm,5μm),流动相为乙腈-0.05 mol·L-1磷酸二氢钠溶液(35∶65),检测波长为440 nm,流速为1.0 ml·min-1。柱温为30℃。结果:血竭素线性范围为0.047~0.234μg,r=0.999 9,平均加样回收率为99.9%,RSD为1.5%。结论:本法操作简便、快速、准确可靠,专属性强,可作为骨伤跌打止痛胶囊的质量控制方法。
Objective: To establish a method for determining the content of Dracorhodin in Gujian Daddy Zhitong Capsule. Methods: The HPLC method was adopted. The mobile phase consisted of Shimadzu VP-ODS C18 (250 mm × 4.6 mm, 5 μm) with a mobile phase of acetonitrile-0.05 mol·L-1 sodium phosphate monobasic (35:65) , The flow rate of 1.0 ml · min-1. The column temperature was 30 ° C. Results: The linear range of DDZ was 0.047 ~ 0.234μg, r = 0.999 9, the average recovery was 99.9% and the RSD was 1.5%. Conclusion: This method is simple, rapid, accurate, reliable and specific. It can be used as a quality control method for Zhuangjian Dadao Zhitong Capsule.