论文部分内容阅读
[目的]研究一起人间肺鼠疫暴发流行的鼠疫菌分离株其生物学特性、基因特征等。拟从分子流行病学的角度分析传染源和传播关系。[方法]对8株于鼠疫患者(尸体)分离的鼠疫菌进行糖醇类酵解试验;检测其毒力、毒力因子、质粒、生物型、基因型;运用分子流行病学方法分析本次流行的传染源。[结果]8株鼠疫菌全部为甘油+、阿胶糖+、脱氮+,生物型为古典型。具备强毒鼠疫菌的4个毒力决定因子,为FraI+、VW+、PstI+、Pgm+;对小白鼠的最小致死量介于50～1000个菌,为强毒株;具备我国多数鼠疫菌所携带的6、45和65Mdal质粒;基因组型全部为10型。[结论]根据分子流行病学和现场流行病学调查结果证实,此次肺鼠疫暴发流行来自同一个传染源。
[Objective] The research aimed to study the biological characteristics and genetic characteristics of Yersinia pestis isolates that are associated with the outbreak of human pulmonary plague. We intend to analyze the relationship between infection source and transmission from the perspective of molecular epidemiology. [Method] Eight strains of Yersinia pestis isolates from plague plague patients were subjected to sugar alcoholic glycolysis test. Their virulence, virulence factors, plasmids, biotypes and genotypes were tested. The molecular epidemiological analysis Popular source of infection. [Result] All the strains of Yersinia pestis were glycerol +, gum arabic +, denitrifying +, and the biological type was the classic type. The four virulence determinants of virulent Yersinia pestis are FraI +, VW +, PstI + and Pgm +. The minimum lethal dose to mice is between 50 and 1000, which is a virulent strain. 6, 45 and 65Mdal plasmids; all genotypes were type 10. [Conclusion] According to the results of molecular epidemiology and field epidemiological investigation, the outbreak of pneumonic plague originated from the same source of infection.