目的:改进盐酸艾司洛尔注射液含量测定的HPLC方法.方法:采用HPLC法,色谱柱为Welch Xltimate C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-甲醇-磷酸盐缓冲液(15:20:65),检测波长为222 nm,流速为1.0 ml·min-1,柱温为30℃,进样体积为20μl.结果:盐酸艾司洛尔的浓度范围在12.26～196.16μg·ml-1时,峰面积与浓度呈良好的线性关系(r=1.0000);平均回收率为101.2％(RSD=0.7％,n=9).结论:建立的盐酸艾司洛尔注射液HPLC简便、快速、准确、专属性强,比现行标准更能反应药物含量的真实水平,更适用于盐酸艾司洛尔注射液的含量测定.“,”Objective:To improve the HPLC method for the determination of esmolol hydrochloride injection .Methods: HPLC was used and the column was a Welch Xltimate C 18 column(250 mm ×4.6 mm, 5μm).The mobile phase was acetonitrile -methanol-phosphate buffer (15:20:65), and the detection wavelength was 222 nm.The flow rate was 1.0 ml· min-1, the column temperature was 30℃, and the sample volume was 20μl.Results:The concentration of esmolol hydrochloride showed a good linear relationship ( r=1.0000) with the peak area within the range of 12.26-196.16 μg· ml-1, and the average recovery was 101.2％(RSD=0.7％, n=9).Conclusion:The method is simple, rapid, accurate and reproducible , which can show truer drug content when compared with the current standard , and is more suitable for the content determination of esmolol hydrochloride injection .