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为证实婴儿肺炎中沙眼衣原体的感染，应用ＨｅＬａ－２２９细胞对４９例肺炎婴儿的鼻咽部分泌物进行沙眼衣原体（ＣＴ）培养分离，以特异的单克隆荧光抗体进行鉴定；同时应用酶联免疫吸附试验检测患儿血清中ＣＴ特异性ＩｇＭ。结果其中９例患儿（最小４天，最大８个月）的标本于荧光显微镜下检测到典型ＣＴ包涵体荧光颗粒、并于相应的血清中检测到高效价ＩｇＭ，检出率为１８．４％（９／４９），其中６个月以下婴儿为２３．５％（８／３４）。９例患儿病初均未采用红霉素治疗，病程迁延最长达４４天，平均２０天。经研究证实，ＣＴ确为婴儿肺炎的重要病原体，故对婴儿肺炎，尤其６个月以下婴儿肺炎，应高度注意ＣＴ感染的可能；早期确诊、及时应用ＣＴ敏感药物，方能取得较好疗效。
In order to confirm the infection of Chlamydia trachomatis in infantile pneumonia, nasopharyngeal secretions from 49 pneumonia infants were isolated and cultured with HeLa-229 cells and identified by specific monoclonal antibody. Meanwhile, enzyme-linked immunosorbent assay Adsorption test for detection of CT-specific IgM in serum of children. Results In 9 of them (minimum 4 days, maximum 8 months), typical CT inclusion body fluorescent particles were detected under fluorescence microscope and high titer IgM was detected in the corresponding serum, the detection rate was 18.4 % (9/49), of which 23.5% (8/34) were infants under 6 months old. Nine cases of children were not treated with erythromycin early disease duration up to 44 days, an average of 20 days. The study confirmed that CT is indeed an important pathogen of pneumonia in infants, so for infant pneumonia, especially infants under 6 months of pneumonia should pay close attention to the possibility of CT infection; early diagnosis and timely application of CT-sensitive drugs in order to achieve better results.